Development of a process for large-scale chromatographic purification of an alginate lyase from Klebsiella pneumoniae

@article{Aasen2004DevelopmentOA,
  title={Development of a process for large-scale chromatographic purification of an alginate lyase from Klebsiella pneumoniae},
  author={Inga Marie Aasen and Kari Folkvord and David W. Levine},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={37},
  pages={55-60}
}
A process for purification of an alginate lyase, produced extracellularly by fermentation of Klebsiella pneumoniae, has been developed. The process includes two chromatographic steps and is well suited to large-scale operation. By hydrophobic interaction chromatography on Phenyl-Sepharose FF, followed by anion exchange chromatography on Q-Sepharose FF in a negative mode, the specific activity was increased from 0.09 units (U) mg −1 to more than 50 U mg−1. Due to an extremely low product… CONTINUE READING
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