Development of a process for large-scale chromatographic purification of an alginate lyase from Klebsiella pneumoniae

  title={Development of a process for large-scale chromatographic purification of an alginate lyase from Klebsiella pneumoniae},
  author={Inga Marie Aasen and Kari Folkvord and David W. Levine},
  journal={Applied Microbiology and Biotechnology},
A process for purification of an alginate lyase, produced extracellularly by fermentation of Klebsiella pneumoniae, has been developed. The process includes two chromatographic steps and is well suited to large-scale operation. By hydrophobic interaction chromatography on Phenyl-Sepharose FF, followed by anion exchange chromatography on Q-Sepharose FF in a negative mode, the specific activity was increased from 0.09 units (U) mg −1 to more than 50 U mg−1. Due to an extremely low product… CONTINUE READING
6 Citations
16 References
Similar Papers


Publications referenced by this paper.
Showing 1-10 of 16 references

Gibor A (1990b) Preparation of alginate lyases from marine molluscs for protoplast isolation in brown algae

  • C Boyen, B Kloareg, M Polne-Fuller
  • Phycologia
  • 1990

Isolation of protoplasts from marine macroalgae. In: Akatsuka I (ed) Introduction to applied phycology

  • DM Butler, LV Evans, B Kloareg
  • SPB Academic Publishing,
  • 1990
1 Excerpt

Modelling singlecomponent protein adsorption to the cation exchanger S-Sepharose FF

  • HA Chase
  • J Chromatogr
  • 1990

Preparation of guluronate lyase from Pseudomonas alginovora for protoplast isolation in Laminaria

  • C Boyen, Y Bertheau, T Barbeyron, B Kloareg
  • Enzyme Microb Technol
  • 1990

Isolation conditions for high yields of protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae)

  • DM Butler, K Ostgaard, C Boyen, LV Evans, A Jensen, B Kloareg
  • J Exp Bot 40:1237-1246
  • 1989
1 Excerpt

Mass production of protoplasts from Macrocystis pyrifera

  • B Kloareg, M Polne-Fuller, A Gibor
  • Plant Science
  • 1989
1 Excerpt

Process-scale chromatography

  • G Jagschies
  • G (eds) Ullmanns's encyclopedia of industrial…
  • 1988
1 Excerpt

Similar Papers

Loading similar papers…