Development of a procedure for the direct cloning of T-cell epitopes using bacterial expression systems.

@article{Neophytou1996DevelopmentOA,
  title={Development of a procedure for the direct cloning of T-cell epitopes using bacterial expression systems.},
  author={Pavlos O. Neophytou and Patricia Ozegbe and Don Healey and Ruby Quartey-Papafio and Anne Cooke and John C. Hutton},
  journal={Journal of immunological methods},
  year={1996},
  volume={196 1},
  pages={
          63-72
        }
}
Although single bacterial recombinant antigens have been used successfully to stimulate individual T-cell clones and elicit recall responses in peripheral lymphocytes, the broader use of molecular cloning systems for the identification of autoantigens recognised by the cellular arm of the immune system has met with only limited success. In a systematic approach to address this problem, a series of bacterial expression vectors were examined for their potential use as cloning vectors to elicit a… CONTINUE READING