Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering.

@article{Yang2010DevelopmentOA,
  title={Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering.},
  author={Bin Yang and Yifen Zhang and Liuhua Zhou and Zeyu Sun and Junhua Zheng and Yun Chen and Yutian Dai},
  journal={Tissue engineering. Part C, Methods},
  year={2010},
  volume={16 5},
  pages={
          1201-11
        }
}
In this study, we compared four decellularization protocols and finally developed an optimized one through which a porcine bladder acellular matrix (BAM) with well-preserved extracellular bioactive factors had been prepared. In this protocol, the intact bladder was treated with trypsin/ethylenediaminetetraacetic acid to remove the urothelium, then with hypotonic buffer and Triton X-100 in hypertonic buffer to remove the membranous and cytoplasmic materials, and finally with nuclease to degrade… CONTINUE READING
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