Development of a polymerase chain reaction-based assay for the detection of Alternaria fungal contamination in food products.

@article{Zur1999DevelopmentOA,
  title={Development of a polymerase chain reaction-based assay for the detection of Alternaria fungal contamination in food products.},
  author={G Zur and Eric M. Hallerman and Rushda Sharf and Yechezkel Kashi},
  journal={Journal of food protection},
  year={1999},
  volume={62 10},
  pages={
          1191-7
        }
}
Alternaria sp. are important fungal contaminants of vegetable, fruit, and grain products, including Alternaria alternata, a contaminant of tomato products. To date, the Howard method, based on microscopic observation of fungal filaments, has been the standard examination for inspection of tomato products. We report development of a polymerase chain reaction (PCR)-based method for detection of Alternaria DNA. PCR primers were designed to anneal to the internal transcribed regions ITS1 and ITS2… Expand
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