Development of a plasmid-cloning system for Streptomyces viridochromogenes Tü494.

Abstract

A plasmid-cloning system was developed for Streptomyces viridochromogenes Tü494, a producer of the tripeptide antibiotic phosphinothricyl-alanyl-alanine (PTT). Parameters affecting protoplast formation and transformability of S. viridochromogenes were investigated in detail. A procedure giving rise to transformation efficiencies of 10(4)-10(5) transformants… (More)

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@article{Strauch1987DevelopmentOA, title={Development of a plasmid-cloning system for Streptomyces viridochromogenes T{\"{u}494.}, author={Eva M Strauch and Wolfgang Wohlleben and Alfred P{\"{u}hler}, journal={Journal of basic microbiology}, year={1987}, volume={27 8}, pages={449-55} }