Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus.

@article{Cromeans1987DevelopmentOA,
  title={Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus.},
  author={Theresa L Cromeans and Mark D. Sobsey and Howard A Fields},
  journal={Journal of medical virology},
  year={1987},
  volume={22 1},
  pages={45-56}
}
Most hepatitis A virus (HAV) replication in cell culture has been reported to be nonlytic and relatively slow. A rapidly replicating isolate of strain HM-175 from persistently infected, serially passed cell cultures (pHM-175) was found to induce a cytopathic effect. This observation allowed the development of a classic plaque assay for pHM-175 in FRhK-4 cells. The plaques were neutralized by polyclonal and monoclonal antisera to HAV.