Development of a novel substrate capture immunoassay for the detection of a neutral metalloproteinase capable of degrading basement membrane (type IV) collagen.

@article{Wacher1990DevelopmentOA,
  title={Development of a novel substrate capture immunoassay for the detection of a neutral metalloproteinase capable of degrading basement membrane (type IV) collagen.},
  author={M P Wacher and Henry C. Krutzsch and Lance A. Liotta and William G Stetler-Stevenson},
  journal={Journal of immunological methods},
  year={1990},
  volume={126 2},
  pages={
          239-45
        }
}
This paper describes the development of a modified substrate capture immunoassay useful in the detection of a neutral metalloproteinase enzyme, type IV collagenase. Capture and immobilization of this enzyme was achieved by coating the microtiter plate with gelatin, an alternative substrate for this enzyme. The captured enzyme could then be detected by affinity-purified rabbit anti-type IV collagenase antibodies prepared against synthetic peptides from the amino terminus of the enzyme. Soluble… CONTINUE READING
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