Development of a novel quantitative real-time assay using duplex scorpion primer for detection of Chlamydia trachomatis.

Abstract

A novel quantitative real-time PCR method using the duplex scorpion primer for detection of Chlamydia trachomatis DNA was developed and validated. The assay employs a duplex primer; its most important feature is the intramolecular probe-target interaction. The assay had many prominent characteristics. (i) The duplex probe is simpler to synthesize and… (More)

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@article{Xia2007DevelopmentOA, title={Development of a novel quantitative real-time assay using duplex scorpion primer for detection of Chlamydia trachomatis.}, author={Qian-feng Xia and Song-xiao Xu and Dong-Sheng Wang and Yang-an Wen and Xi Qin and Shi-Yun Qian and Zhi-Long Zhan and Hua-ming Wang and Yin-Zhi Lin and Zhi-guang Tu}, journal={Experimental and molecular pathology}, year={2007}, volume={83 1}, pages={119-24} }