Development of a novel one-tube isothermal reverse transcription thermophilic helicase-dependent amplification platform for rapid RNA detection.

@article{Goldmeyer2007DevelopmentOA,
  title={Development of a novel one-tube isothermal reverse transcription thermophilic helicase-dependent amplification platform for rapid RNA detection.},
  author={James Goldmeyer and Huimin Kong and Wen Tang},
  journal={The Journal of molecular diagnostics : JMD},
  year={2007},
  volume={9 5},
  pages={639-44}
}
The high complexity and cost of polymerase chain reaction-based molecular diagnostics sometimes limits their use in the clinical diagnostics setting. A new helicase-based isothermal amplification method offers an alternative to standard polymerase chain reaction, allowing amplification and detection of specific DNA sequences at a constant reaction… CONTINUE READING