Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors.

@article{Kawaguchi2008DevelopmentOA,
  title={Development of a novel fluorescent probe for fluorescence correlation spectroscopic detection of kinase inhibitors.},
  author={Mitsuyasu Kawaguchi and Takuya Terai and Rei Utata and Miki Kato and Keiko Tsuganezawa and Akiko Tanaka and Hirotatsu Kojima and Takayoshi Okabe and T. SonyEricssonMobileCommunicationsJapanInc Nagano},
  journal={Bioorganic & medicinal chemistry letters},
  year={2008},
  volume={18 13},
  pages={
          3752-5
        }
}
We have developed a fluorescently labeled probe for high-throughput screening of kinase inhibitors using fluorescence correlation spectroscopy. With this probe, we have successfully evaluated the inhibitory activities of known inhibitors of a model kinase, ASK1. Because the probe contains a general kinase inhibitor, staurosporine, we believe that this homogeneous, high-throughput, and simple method can be applied to the inhibitor screening of other kinases as well. 
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