Development of a novel electrophoretic system for the isolation of human spermatozoa.

@article{Ainsworth2005DevelopmentOA,
  title={Development of a novel electrophoretic system for the isolation of human spermatozoa.},
  author={Christopher J Ainsworth and Brett Nixon and Robert John Aitken},
  journal={Human reproduction},
  year={2005},
  volume={20 8},
  pages={2261-70}
}
BACKGROUND Optimization of assisted conception outcomes involves the development of rapid, safe, effective techniques for the isolation of functional human spermatozoa free from significant DNA damage. In this study we describe a novel electrophoretic sperm isolation technique that achieves these objectives. METHODS The separation system consisted of a cassette comprising two 400 mul chambers separated by a polycarbonate filter containing 5 micromol/l pores and bounded by a 15 kDa… CONTINUE READING

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