Development of a novel dengue virus serotype‐specific multiplex real‐time reverse transcription–polymerase chain reaction assay for blood screening

@article{Tezuka2016DevelopmentOA,
  title={Development of a novel dengue virus serotype‐specific multiplex real‐time reverse transcription–polymerase chain reaction assay for blood screening},
  author={Kenta Tezuka and Madoka Kuramitsu and Kazu Okuma and Kiyoko Nojima and Kumiko Araki and Naoya Shinohara and Chieko Matsumoto and Masahiro Satake and Tomohiko Takasaki and Masayuki Saijo and Ichiro Kurane and Isao Hamaguchi},
  journal={Transfusion},
  year={2016},
  volume={56}
}
Dengue fever is caused by four related RNA viruses of the genus Flavivirus, dengue virus (DENV)‐1, ‐2, ‐3, and ‐4, which are transmitted to humans by mosquitoes. Although DENV is not endemic in Japan, an autochthonous dengue outbreak occurred in 2014. Several transfusion‐transmitted cases have also been reported after the use of blood and plasma products in DENV‐endemic countries. The aim of this study was to develop a novel multiplex reverse transcription–polymerase chain reaction (RT‐PCR… 
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A multiplex quantitative polymerase chain reaction (qPCR) by large‐scale primer screening was developed and can successfully and simultaneously detect both types of HTLV‐1 andHTLV‐2 provirus with extremely high sensitivity.
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TLDR
RVSVs that specifically target and kill HTLV-1 Env-expressing cells (not ATL cells, which generally do not express Env in vivo) through replacement of the G gene with HT LV-1 receptor gene(s) in the VSV genome are developed.

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