Development of a novel assay for human tyrosyl DNA phosphodiesterase 2.

@article{Adhikari2011DevelopmentOA,
  title={Development of a novel assay for human tyrosyl DNA phosphodiesterase 2.},
  author={Sanjay Adhikari and Soumendra Krishna Karmahapatra and Hadi Elias and Priyanka Dhopeshwarkar and R Scott Williams and Stephen J Byers and Aykut Uren and Rabindra K. Roy},
  journal={Analytical biochemistry},
  year={2011},
  volume={416 1},
  pages={112-6}
}
Tyrosyl DNA phosphodiesterase 2 (TDP2), a newly discovered enzyme that cleaves 5'-phosphotyrosyl bonds, is a potential target for chemotherapy. TDP2 possesses both 3'- and 5'-tyrosyl-DNA phosphodiesterase activity, which is generally measured in a gel-based assay using 3'- and 5'-phosphotyrosyl linkage at the 3' and 5' ends of an oligonucleotide. To understand the enzymatic mechanism of this novel enzyme, the gel-based assay is useful, but this technique is cumbersome for TDP2 inhibitor… CONTINUE READING