Development of a novel ER stress based selection system for the isolation of highly productive clones

  title={Development of a novel ER stress based selection system for the isolation of highly productive clones},
  author={L. K{\o}ber and Christoph Zehe and J. Bode},
  journal={Biotechnology and Bioengineering},
  • L. Køber, Christoph Zehe, J. Bode
  • Published 2012
  • Biology, Medicine
  • Biotechnology and Bioengineering
  • Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of calreticulin (CALR), glucose‐regulated protein 78 and 94 kDa (GRP78, GRP94) and spliced X‐box binding protein 1 (XPB1) was observed. By means of these results we were motivated to… CONTINUE READING
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