Development of a novel Borna disease virus reverse genetics system using RNA polymerase II promoter and SV40 nuclear import signal.

@article{Yanai2006DevelopmentOA,
  title={Development of a novel Borna disease virus reverse genetics system using RNA polymerase II promoter and SV40 nuclear import signal.},
  author={Hideyuki Yanai and Yohei Hayashi and Yohei Watanabe and Naohiro Ohtaki and Takeshi Kobayashi and Yasutoshi Nozaki and Kazufumi Ikuta and Keizo Tomonaga},
  journal={Microbes and infection},
  year={2006},
  volume={8 6},
  pages={
          1522-9
        }
}
Borna disease virus (BDV) is a noncytolytic, neurotropic RNA virus that replicates and transcribes in the nucleus of infected cells. Therefore, efficient synthesis of BDV RNA in the nucleus is critical for the development of a reverse genetics system for this virus. Here, we report the development of such a system using the RNA polymerase II (Pol II) promoter. The BDV minigenome cDNA was flanked by hammerhead ribozyme and hepatitis delta ribozyme sequences and inserted downstream of the Pol II… CONTINUE READING

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