Development of a nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum.

@article{Grooters2002DevelopmentOA,
  title={Development of a nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum.},
  author={Amy M Grooters and Melaney K Gee},
  journal={Journal of veterinary internal medicine},
  year={2002},
  volume={16 2},
  pages={147-52}
}
Pythium insidiosum is an important cause of cutaneous and gastrointestinal disease in horses and dogs in the southeastern United States. Culture-based diagnosis of pythiosis is rarely definitive because production and identification of reproductive structures is difficult. The purpose of this study was to develop a polymerase chain reaction (PCR)-based assay for the identification of P insidiosum. Genomic DNA was extracted from 3 clinical isolates of P insidiosum and I isolate each of Pythium… CONTINUE READING

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