Development of a multitarget real-time TaqMan PCR assay for enhanced detection of Francisella tularensis in complex specimens.

@article{Versage2003DevelopmentOA,
  title={Development of a multitarget real-time TaqMan PCR assay for enhanced detection of Francisella tularensis in complex specimens.},
  author={Jessica L. Versage and Darlena D M Severin and Min Ying Chu and Jeannine M. Petersen},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 12},
  pages={
          5492-9
        }
}
Tularemia is the zoonotic disease caused by the gram-negative coccobacillus Francisella tularensis. Its wide distribution in the environment poses a challenge for understanding the transmission, ecology, and epidemiology of the disease. F. tularensis is also considered a potential biological weapon due to its extreme infectivity. We have developed a multitarget real-time TaqMan PCR assay capable of rapidly and accurately detecting F. tularensis in complex specimens. Targeted regions included… CONTINUE READING
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