Development of a multiplex real-time PCR for contagious agalactia diagnosis in small ruminants.

  title={Development of a multiplex real-time PCR for contagious agalactia diagnosis in small ruminants.},
  author={Claire A.M. Becker and Fabien Ramos and Eric Sellal and Sandrine Moine and François Poumarat and Florence Tardy},
  journal={Journal of microbiological methods},
  volume={90 2},
Contagious agalactia is an important disease worldwide that affects small ruminants. Clinical manifestations vary from mastitis, pneumonia, arthritis and keratoconjunctivitis to septicemia. Four mycoplasmal etiological agents have been identified: Mycoplasma (M.) agalactiae, M. mycoides subsp. capri, M. capricolum subsp. capricolum and M. putrefaciens. The current procedure for direct diagnosis, recommended by the World Organization for Animal Health, involves the isolation of one or several… CONTINUE READING


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