Development of a multiplex polymerase chain reaction for the differentiation of bovine herpesvirus-1 and -5.

@article{Alegre2001DevelopmentOA,
  title={Development of a multiplex polymerase chain reaction for the differentiation of bovine herpesvirus-1 and -5.},
  author={Mariana Alegre and Mariana Nanni and Norberto Fondevila},
  journal={Journal of veterinary medicine. B, Infectious diseases and veterinary public health},
  year={2001},
  volume={48 8},
  pages={613-21}
}
Bovine herpesvirus-1 (BHV-1) and bovine herpesvirus-5 (BHV-5) are closely related viruses which exhibit some important differences at the genetic and immunogenic levels which may explain the differences in their pathogenicity and epidemiological characteristics. A multiplex polymerase chain reaction (M-PCR) was developed to detect and differentiate between BHV-1 and BHV-5. In this M-PCR two pairs of primers (TK1, TK2 and GD1, GD2) were used in the same reaction mix to amplify a thymidine kinase… CONTINUE READING

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