Development of a multiplex PCR for identification of vineyard mealybugs.

  title={Development of a multiplex PCR for identification of vineyard mealybugs.},
  author={Kent M Daane and Mathew C Middleton and Ren{\'e} François Henri Sforza and Monica L. Cooper and Vaughn M. Walton and Douglas Bruce Walsh and Tania Zaviezo and Rodrigo P P Almeida},
  journal={Environmental entomology},
  volume={40 6},
A simple molecular tool was developed and tested to identify seven mealybug species found in North American vineyards: Pseudococcus maritimus Ehrhorn, Pseudococcus viburni (Signoret), Pseudococcus longispinus (Targioni-Tozzeti), Pseudococcus calceolariae (Maskell), Planococcus ficus (Signoret), Planococcus citri (Risso), and Ferrisia gilli Gullan. The developed multiplex PCR is based on the mitochondrial cytochrome c oxidase subunit one gene. In tests, this single-step multiplex PCR correctly… CONTINUE READING
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