Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains.

@article{Ooka2009DevelopmentOA,
  title={Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains.},
  author={Tadasuke Ooka and Jun Terajima and Masahiro Kusumoto and Atsushi Iguchi and Ken Kurokawa and Yoshitoshi Ogura and Md Asadulghani and Keisuke Nakayama and Kazunori Murase and Makoto Ohnishi and Sunao Iyoda and Haruo Watanabe and Tetsuya Hayashi},
  journal={Journal of clinical microbiology},
  year={2009},
  volume={47 9},
  pages={2888-94}
}
Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods is crucial for the rapid detection and surveillance of EHEC O157 outbreaks. In the present study, we developed a multiplex PCR-based strain-typing method for EHEC O157, which is based on the variability in genomic location of IS629 among EHEC O157 strains. This method is very simple, in that the procedures are… CONTINUE READING

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Inference of the impact of insertion sequence ( IS ) elements on bacterial genome diversification through analysis of small - size structural polymorphisms in Escherichia coli O 157 genomes

J. T. Riordan, S. B. Viswanath, S. D. Manning, T. S. Whittam
Genome Res • 2008

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