Development of a monoclonal antibody specific to a recombinant envelope protein from dengue virus type 4 expressed in Pichia pastoris.

Abstract

A mouse monoclonal antibody (MAb, 4B6) was able to recognize dengue virus type 4 envelope (E) protein both as a recombinant protein in Pichia pastoris and when it was present in infected brains of suckling mice. 4B6 was characterized by enzyme-linked immunoadsorbent assay (ELISA), hemaglutination inhibition, neutralization, and immunoblot. The MAb was isotyped as IgG2a. It was serotype 4 specific and it inhibited hemaglutination and neutralized homologous virus. It did not enhance infection of P338D1 cells by dengue type 4 virus strain H-241 strain. This MAb was reactive with recombinant E protein and dengue 4 virus, as revealed by Western blot. In vivo, MAb 4B6 conferred passive protection in mice challenged with homologous virus. Currently, this MAb is being used to purify recombinant E protein for further studies.

Cite this paper

@article{PupoAntnez2001DevelopmentOA, title={Development of a monoclonal antibody specific to a recombinant envelope protein from dengue virus type 4 expressed in Pichia pastoris.}, author={Maritza Pupo-Ant{\'u}nez and Rosal{\'i}a Hillers Rodriguez and Mercedes {\'A}lvarez and Nayab Amin and Hilze Rodr{\'i}guez and Anselmo J Otero and Gabriel S{\'a}nchez de Guzm{\'a}n}, journal={Hybridoma}, year={2001}, volume={20 1}, pages={35-41} }