Development of a monoclonal antibody-based cELISA for the analysis of sulfadimethoxine. 1. Development and characterization of monoclonal antibodies and molecular modeling studies of antibody recognition.

@article{Muldoon2000DevelopmentOA,
  title={Development of a monoclonal antibody-based cELISA for the analysis of sulfadimethoxine. 1. Development and characterization of monoclonal antibodies and molecular modeling studies of antibody recognition.},
  author={Molly Muldoon and Carol K. Holtzapple and Suvarna S. Deshpande and Ross C. Beier and Larry Henry Stanker},
  journal={Journal of agricultural and food chemistry},
  year={2000},
  volume={48 2},
  pages={
          537-44
        }
}
Sulfonamide antibiotics are used to treat a variety of bacterial and protozoan infections in cattle, swine, and poultry. Current residue methods for the analysis of sulfonamides in animal-based food products include bioassays, chromatographic methods (HPLC, GLC), and immunoassays. Most immunoassays have employed highly specific polyclonal antibodies. In this paper, we describe the isolation of monoclonal antibodies against sulfadimethoxine (SDM) that vary in their sensitivities and cross… CONTINUE READING
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