Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells

  title={Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells},
  author={Shin Yoshioka and Wataru Fujii and Tetsuhiro Ogawa and Koji Sugiura and Kunihiko Naito},
  booktitle={Scientific reports},
The CRISPR/Cas9 system has been used for spatio-temporal gene modification through the ubiquitous expression of gRNA by an RNA polymerase III promoter and the controlled expression of Cas9 using a tissue-specific or inducible promoter. However, unexpected gene disruptions indicate the necessity of a tissue-specific or inducible expression of not only Cas9 but also gRNA. In the present study, we attempted to develop a CRISPR/Cas9 system that could express functional gRNAs and Cas9 by a single… CONTINUE READING
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