Development of a method for the measurement of primary cilia length in 3D

  title={Development of a method for the measurement of primary cilia length in 3D},
  author={Taryn Saggese and Alistair A. Young and Chaobo Huang and Kevin Braeckmans and Susan Read McGlashan},
Primary cilia length is an important measure of cell and tissue function. While accurate length measurements can be calculated from cells in 2D culture, measurements in tissue or 3D culture are inherently difficult due to optical distortions. This study uses a novel combination of image processing techniques to rectify optical distortions and accurately measure cilia length from 3D images. Point spread functions and experimental resolutions were calculated from subresolution microspheres… CONTINUE READING
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