Development of a method for recovering rickettsial RNA from infected cells to analyze gene expression profiling of obligate intracellular bacteria.

@article{La2007DevelopmentOA,
  title={Development of a method for recovering rickettsial RNA from infected cells to analyze gene expression profiling of obligate intracellular bacteria.},
  author={My-Van La and Patrice François and Clarisse Rovery and Sylvianne Robineau and Pascal Barbry and Jacques Schrenzel and Didier Raoult and Patricia Renesto},
  journal={Journal of microbiological methods},
  year={2007},
  volume={71 3},
  pages={292-7}
}
The Rickettsia genus is composed of Gram-negative bacteria responsible for Typhus and spotted fevers. Because of the limitations imposed by their obligate intracellular location, the molecular mechanisms responsible for their pathogenicity remain poorly understood. Several rickettsial genomes are now available, thus providing the foundation for a new era of post-genomic research. Here, using Rickettsia conorii as model, we developed a suitable method for microarray-based transcriptome analysis… CONTINUE READING
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