Development of a method for markerless gene deletion in Pseudomonas putida.

@article{Graf2011DevelopmentOA,
  title={Development of a method for markerless gene deletion in Pseudomonas putida.},
  author={Nadja Graf and Josef Altenbuchner},
  journal={Applied and environmental microbiology},
  year={2011},
  volume={77 15},
  pages={5549-52}
}
We developed a negative counterselection system for Pseudomonas putida based on uracil phosphoribosyltransferase (UPRTase) and sensitivity against the antimetabolite 5-fluorouracil (5-FU). We constructed a P. putida strain that is resistant to 5-FU and constructed vectors for the deletion of the surface adhesion protein gene, the flagellum biosynthesis operon, and two endonuclease genes. The genes were efficiently disrupted and left a markerless chromosomal in-frame deletion. 
Related Discussions
This paper has been referenced on Twitter 1 time. VIEW TWEETS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 23 extracted citations

Genetic tools for reliable gene expression and recombineering in Pseudomonas putida

Journal of Industrial Microbiology & Biotechnology • 2017
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…