Development of a method employing reversed-phase thin-layer chromatography for establishing milk fat purity with respect to adulteration with vegetable oils

@article{Upadhyay2015DevelopmentOA,
  title={Development of a method employing reversed-phase thin-layer chromatography for establishing milk fat purity with respect to adulteration with vegetable oils},
  author={Neelam Upadhyay and Anil Kumar and Gopal Rathod and Ankit Goyal and Darshan Lal},
  journal={International Journal of Dairy Technology},
  year={2015},
  volume={68},
  pages={207-217}
}
A systematic study was conducted using reversed-phase thin-layer chromatography wherein unsaponifiable matter of samples namely, pure cow and buffalo milk fats, vegetable oils (groundnut oil, soya bean oil and sunflower oil) and milk fats adulterated with vegetable oils (≥1%) were run along with the reference standards using two new solvent systems to detect adulteration in milk fat. The results of the study revealed that adulteration at even a 1% level could easily be detected using this rapid… 
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References

SHOWING 1-10 OF 23 REFERENCES

Detection of milk fat adulteration with admixture of foreign oils and fats using a fractionation technique and the apparent solidification time test

A fractionation technique followed by the apparent solidification time (AST) test was adopted for detecting the admixture of foreign oils and fats in milk fat. The AST values of the solid fraction…

Chromatographic analysis of plant sterols in foods and vegetable oils.

  • S. Abidi
  • Chemistry, Medicine
    Journal of chromatography. A
  • 2001

An approach to the detection of synthetic milk in dairy milk: 3. Detection of vegetable oil and sodium bicarbonate

A high-performance thin layer chromatography (HPTLC) method for the detection of groundnut oil and palm oil, the main constituents of synthetic milk, was developed. The minimum detectable level of…

Development of steryl ester analysis for the detection of admixtures of vegetable oils

The steryl ester content and composition of 28 samples from 10 vegetable oil types have been determined by isolation of the steryl esters by high-performance liquid chromatography and analysis by gas…

Authentication of milk and milk-based foods by direct analysis in real time ionization–high resolution mass spectrometry (DART–HRMS) technique: A critical assessment

Content and distribution oftrans-18:1 acids in ruminant milk and meat fats. Their importance in european diets and their effect on human milk

Thetrans-18:1 acid content and distribution in fats from ewe and goat milk, beef meat and tallow were determined by a combination of capillary gas-liquid chromatography and argentation thin-layer…

Qualitative and quantitative analysis of the unsaponifiable fraction of vegetable oils by using comprehensive 2D GC with dual MS/FID detection

The present investigation is focused on the development of a comprehensive two-dimensional GC (GC × GC) method, with dual MS/FID detection, for the qualitative and quantitative analysis of the entire…

Milk fat components : possible chemopreventive agents for cancer and other diseases

Milk fat contains a number of components such as sphingomyelin, conjugated linoleic acid, butyric acid, ether lipids, vitamin A, β-carotene and vitamin D which have the potential to inhibit the…

Tocopherol, tocotrienol and plant sterol contents of vegetable oils and industrial fats

Milk fat in human nutrition

The review discusses the antimicrobial action of milk fat and its role in long-chain polyunsaturated fatty acid metabolism, bone growth, ulcerogenesis, and energy metabolism.