Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency.

@article{Keller2009DevelopmentOA,
  title={Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency.},
  author={Kimberly L. Keller and Kelly S. Bender and Judy D Wall},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 24},
  pages={7682-91}
}
In recent years, the genetic manipulation of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough has seen enormous progress. In spite of this progress, the current marker exchange deletion method does not allow for easy selection of multiple sequential gene deletions in a single strain because of the limited number of selectable markers available in D. vulgaris. To broaden the repertoire of genetic tools for manipulation, an in-frame, markerless deletion system has been… CONTINUE READING

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