Development of a lymphocyte transformation microassay for murine leukemia virus.

@article{Donahoe1976DevelopmentOA,
  title={Development of a lymphocyte transformation microassay for murine leukemia virus.},
  author={Robert M. Donahoe and Robert L. Peters and R VanVleck and Robert J. Huebner and Gary j. Kelloff},
  journal={Journal of the National Cancer Institute},
  year={1976},
  volume={56 1},
  pages={51-7}
}
A lymphocyte transformation microassay (LTA) was developed from spleen harvests of 6- to 8-week-old BABL/cCr mice. The optimal culture conditions for the microassay were established by measurement of lymphoblastogenesis in response to phytohemagglutin (PHA) and pokeweek mitogen. Immunization, as measured by the LTA, of adult BALB/cCr mice with formalin… CONTINUE READING