Development of a low capital investment reactor system: application for plant cell suspension culture

@article{Hsiao1999DevelopmentOA,
  title={Development of a low capital investment reactor system: application for plant cell suspension culture},
  author={Hsiao and Bacani and Carvalho and Curtis},
  journal={Biotechnology progress},
  year={1999},
  volume={15 1},
  pages={114-22}
}
Growth of plant cell cultures is demonstrated in an uncontrolled, simple, and inexpensive plastic-lined vessel. Sustained specific growth rates of 0.22 day-1 for Hyoscyamus muticus cell suspension cultures are achieved in a low-cost gas-sparged bioreactor configuration (6.5 L working volume, wv) which is comparable to an "optimized" 5 L wv mechanically agitated fermentor. In an effort to reduce bioreactor costs, the need for an autoclavable vessel was eliminated. Sterilization is achieved by… CONTINUE READING

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