Development of a live tissue microtome: reflections of an amateur machinist

  title={Development of a live tissue microtome: reflections of an amateur machinist},
  author={Carlos L. Krumdieck},
  pages={2 - 7}
Live circular tissue slices of nearly identical diameter and thickness can be generated from most tissues by the use of two instruments; a coring tool to cut cylindrical tissue cores which are subsequently sliced by a microtome into thin circular sections 5–8 mm in diameter and 50–300 microns thick. The sections are of very similar geometry permitting direct comparisons without normalization. Both instruments operate submerged in a cold isotonic medium that caries the cut slices outside the… 
Laser Microdissection of the Alveolar Duct Enables Single-Cell Genomic Analysis
An approach to conduct laser microdissection of the lung alveolar duct for single-cell PCR analysis is demonstrated and preliminary work suggests the unique value of this approach to understand the intra- and intercellular interactions within the regenerating alveolars duct.
Optimization and comprehensive characterization of a faithful tissue culture model of the benign and malignant human prostate
A TSC model is demonstrated that authentically recapitulates the structural, cellular, and genetic characteristics of the benign and malignant human prostate, androgen dependence of the native tissue, and cancer-specific response to a potentially new therapeutic for PCa.
Evaluation of the metabolism and hepatotoxicity of xenobiotics utilizing precision-cut slices
  • B. Lake, R. J. Price
  • Biology
    Xenobiotica; the fate of foreign compounds in biological systems
  • 2013
Precision-cut liver slices can be utilized to examine species differences in hepatic xenobiotic metabolism and xenobiotics-induced toxicity, thus permitting comparisons between animal species and humans.
Precision-Cut Tumor Slices (PCTS) as an Ex Vivo Model in Immunotherapy Research
PCTS are expected to gain momentum and be fully utilized as a significant tool towards better patient stratification and personalized medicine and the advantages and limitations of the PCTS models are discussed.
Ex vivo tumor culture systems for functional drug testing and therapy response prediction
This review discusses a broad spectrum of model systems, ranging from classic 2D monolayer culture techniques to more experimental ‘cancer-on-chip’ procedures, that take tumor heterogeneity and tumor–stromal interactions into account.
Inhibition of Mouse Breast Tumor-Initiating Cells by Calcitriol and Dietary Vitamin D
The findings indicate that vitamin D compounds target breast TICs reducing tumor-initiating activity and suggest that combining Vitamin D compounds with standard therapies may enhance anticancer activity and improve therapeutic outcomes.
Assessment of the pulmonary CYP1A1 metabolism of mavoglurant (AFQ056) in rat
P phenotyping results indicate that CYP1A1 is responsible for the formation of the oxidative metabolite, M3, and in vivo experiments where intravenous and intra-arterial dosing to rats failed to show significant pulmonary extraction, it was concluded that the reduced M3 formation was due to CYP 1A1 inhibition.
Unravelling lung cancer heterogeneity and associated therapeutic responses using in vivo and ex vivo model systems
This review focuses on the literature on the cell of origin of lung cancer and the role of ERBB family receptors, ligands and their roles in cancer in these studies.


Versuche an Überlebendem Carcinomgewebe
Tissue slicing
  • 1987
Tissue slicing and culturing revisited
Tissue slice technique
  • Method Enzymol
  • 1955
Tissue slice technique
  • Method Enzymol
  • 1955
[1] Tissue slice technique
tissue slices for respiratory experiments
  • J Physiol 87:56P. Elliott KAC
  • 1955
An improvement of Warburg ’ s method for cutting tissue slices for respiratory experiments
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Respiration and functional activity