Development of a liquid chromatography-based screening methodology for proteolytic enzyme activity.


A new methodology for the detection and isolation of serine proteases in complex mixtures has been developed. It combines the characterization of crude samples by electrospray tandem mass spectrometry (ESI-MS/MS) in a multi-substrate assay and the differentiated sensitive detection of the responsible enzymes by means of liquid chromatography hyphenated… (More)
DOI: 10.1016/j.chroma.2009.03.053


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