Development of a lectin binding assay to differentiate between recombinant and endogenous proteins in pharmacokinetic studies of protein-biopharmaceuticals.

@article{Weber2015DevelopmentOA,
  title={Development of a lectin binding assay to differentiate between recombinant and endogenous proteins in pharmacokinetic studies of protein-biopharmaceuticals.},
  author={Alfred P. Weber and Eva Minibeck and Friedrich Scheiflinger and Peter L Turecek},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2015},
  volume={108},
  pages={21-8}
}
Human glycoproteins, expressed in hamster cell lines, show similar glycosylation patterns to naturally occurring human molecules except for a minute difference in the linkage of terminal sialic acid: both cell types lack α2,6-galactosyl-sialyltransferase, abundantly expressed in human hepatocytes and responsible for the α2,6-sialylation of circulating… CONTINUE READING