Development of a highly sensitive lateral immunochromatographic assay for rapid detection of Vibrio parahaemolyticus.

@article{Liu2016DevelopmentOA,
  title={Development of a highly sensitive lateral immunochromatographic assay for rapid detection of Vibrio parahaemolyticus.},
  author={Xinfeng Liu and Yuyao Guan and Shiliang Cheng and Yidan Huang and Qin Yan and Jun Zhang and Guanjun Huang and Jian Zheng and Tianqiang Liu},
  journal={Journal of microbiological methods},
  year={2016},
  volume={131},
  pages={
          78-84
        }
}
Vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. It is also a foodborne pathogen that causes diarrhea in humans. The aim of this study is to develop an immunochromatographic lateral flow assay (LFA) for rapid detection of V. parahaemolyticus in both aquatic products and human feces of diarrheal patients. Two monoclonal antibody (MAb) pairs, GA1a-IC9 and IC9-KB4c, were developed and proven to be highly specific and… 
Development of a monoclonal antibody for specific detection of Vibrio parahaemolyticus and analysis of its antigen.
TLDR
Bioinformatics research demonstrated that the OM lipoprotein is an ortholog of the lpp protein conserved throughout many bacteria, suggesting it may be a good target for detection of V. parahaemolyticus.
Rapid visualized isothermal nucleic acid testing of Vibrio parahaemolyticus by polymerase spiral reaction
TLDR
It is expected that the novel, effortless and reliable isothermal nucleic acid testing assay developed in this study has potential to be applied to screening for V. parahaemolyticus in seafood samples.
Molecular Methods to Study Vibrio parahaemolyticus and Vibrio vulnificus From Atypical Environments
TLDR
This chapter outlines the conventional and molecular methods to identify these two formidable pathogens that remain a global risk.
Detection of microorganisms with lateral flow test strips
TLDR
This chapter provides an overview of the recent research trends in LFIA, i.e., improvement of sensitivity, multimerization, adjustment of specificity, automation of multi-step reaction, quantification, and construction of strip with one type of antibody.
A Dual-Mode (Fluorometric and Colorimetric) Aptasensor for Vibrio parahaemolyticus Detection Using Multifunctional Nanoparticles
Reliable detection of foodborne pathogen is of great importance in guaranteeing food safety. In the present work, based on the multifunctional properties of platinum-coated gold nanorods (AuNR@Pt),
Comparison of 4 label-based immunochromatographic assays for the detection of Escherichia coli O157:H7 in milk.
TLDR
The FNP and EuNP-ICA showed remarkable potential for detection of pathogens in milk and showed better tolerance than GNP-ICA and QD-ICA in the milk samples.
Pathogen Immunoassay in Food
TLDR
The criteria of foodborne pathogens in food was described, the most effective immunoassays among the previous studies for the leadingFoodborne pathogens including Salmonella spp.
Vibrio and major commercially important vibriosis diseases in decapod crustaceans.
TLDR
This review aims to inform and update the reader about the current state of knowledge about Vibrio and associated vibriosis in farmed crustaceans (i.e. shrimp, lobster, and crabs) and identify potential knowledge gaps in the literature, which serves as a basis for future research direction.
Biosensing platforms for Vibrio bacteria detection based on whole cell and nucleic acid analysis: A review.
TLDR
In this review, a focus is given on strip test devices, and on optical and electrochemical biosensors developed for Vibrio analysis, with particular attention to sample preparation techniques.
Polydimethylsiloxane Gold Nanoparticle Composite Film as Structure for Aptamer-Based Detection of Vibrio parahaemolyticus by Surface-Enhanced Raman Spectroscopy
This paper investigated a new detection method for food pathogenic bacteria Vibrio parahaemolyticus based on surface-enhanced Raman scattering (SERS). Nanostructures consisting of Aptamer-Au
...
1
2
...

References

SHOWING 1-10 OF 24 REFERENCES
Development and Evaluation of a Colloidal Gold Immunochromatography Strip for Rapid Detection of Vibrio parahaemolyticus in Food
TLDR
The immunochromatographic strip is successful in and suitable for fast detection of V. parahaemolyticus in food and much high application value in food microorganism, and it is worth to be popularized.
Development and Evaluation of a Rapid, Simple, and Sensitive Immunochromatographic Assay To Detect Thermostable Direct Hemolysin Produced by Vibrio parahaemolyticus in Enrichment Cultures of Stool Specimens
TLDR
TDH-ICA is a rapid, simple, and sensitive TDH detection method and that TDH-ica testing of a fecal enrichment culture is useful as an adjunct to facilitate the early diagnosis of V. parahaemolyticus diarrhea.
An immunochromatographic test strip for rapid detection of fish pathogen Edwardsiella tarda
TLDR
The immunochromatographic test strip offers great promise for a rapid, simple, and economical method of E. tarda on-site detection, and with different antibodies, it might be used to detect other aquatic pathogens.
Characteristics of a sharp decrease in Vibrio parahaemolyticus infections and seafood contamination in Japan.
TLDR
It was suggested that the change of seafood contamination was unrelated to the sharp decrease in V. parahaemolyticus infections, and infections might be prevented at the stages after the distribution stage.
Improved Method for Detection of Vibrio parahaemolyticus in Seafood
TLDR
A new, effective procedure for detecting V. parahaemolyticus in seafoods using enrichment and plating onto a chromogenic agar medium, which was more effective than the one-step enrichment in salt polymyxin broth alone.
A simple and rapid immunochromatographic test strip for detection of pathogenic isolates of Vibrio harveyi.
TLDR
Mouse monoclonal antibodies and rabbit polyclonal antibody against Vibrio harveyi were generated from immunization of mice and rabbits with highly virulent isolate of V.Harveyi and the beneficial features of this kit are that simple, convenient and quick results can be obtained without the requirement of sophisticated tools or special equipments and skills.
Improved Detection of Five Major Gastrointestinal Pathogens by Use of a Molecular Screening Approach
TLDR
An MSA for detection of gastrointestinal pathogens resulted in markedly improved detection rates and a substantial decrease in time to reporting of (preliminary) results.
A collagenase-targeted multiplex PCR assay for identification of Vibrio alginolyticus, Vibrio cholerae, and Vibrio parahaemolyticus.
TLDR
The results of this study indicate that the gene coding for collagenase should be used as an alternative molecular target to discriminate among the three Vibrio species.
Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan.
TLDR
The difference in detection between theMPN-culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors.
Detection of Pathogenic Vibrio spp. in Shellfish by Using Multiplex PCR and DNA Microarrays
TLDR
A gene-specific DNA microarray coupled with multiplex PCR for the comprehensive detection of pathogenic vibrios that are natural inhabitants of warm coastal waters and shellfish and the presence of V. vulnificus and V. parahaemolyticus was revealed in natural oysters.
...
1
2
3
...