Development of a highly sensitive high-performance liquid chromatographic method for measuring an anticancer drug, UCN-01, in human plasma or urine.

@article{Kurata1998DevelopmentOA,
  title={Development of a highly sensitive high-performance liquid chromatographic method for measuring an anticancer drug, UCN-01, in human plasma or urine.},
  author={Noriaki Kurata and Tomoko Kuramitsu and Hiromi Tanii and Eiichi Fuse and Takashi Kuwabara and Hiroyuki Kobayashi and Satoshi Kobayashi},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={708 1-2},
  pages={223-7}
}
We have established a highly sensitive high-performance liquid chromatographic method for the determination of an anticancer drug, UCN-01, in human plasma or urine. Using a fluorescence detector set at an excitation wavelength of 310 nm and emission monitored at 410 nm, there was a good linearity for UCN-01 in human plasma (r=0.999) or urine (r=0.999) at concentrations ranging from 0.2 to 100 ng/ml or 1 to 400 ng/ml, respectively. For intra-day assay, in plasma samples, the precision and… CONTINUE READING