Development of a high-throughput fluorescence polarization assay for Bcl-x(L).

  title={Development of a high-throughput fluorescence polarization assay for Bcl-x(L).},
  author={Haichao Zhang and Paul M. Nimmer and Saul H. Rosenberg and S E Ng and Mary M. Joseph},
  journal={Analytical biochemistry},
  volume={307 1},
Antiapoptotic protein Bcl-x(L) has been demonstrated to play a very important role in a variety of diseases such as cancer. Its biological function can be inhibited by proapoptotic proteins such Bak, Bad, and Bax by forming complexes mediated primarily by the Bcl-2 homology 3 (BH3) domain. To facilitate drug discovery for Bcl-x(L) inhibitors, we have developed and optimized a fluorescence polarization assay based on the interaction between Bcl-x(L) and BH3 domain peptides. We observed that the… CONTINUE READING
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