Development of a high-throughput fluorescence polarization DNA cleavage assay for the identification of FEN1 inhibitors.

@article{McWhirter2013DevelopmentOA,
  title={Development of a high-throughput fluorescence polarization DNA cleavage assay for the identification of FEN1 inhibitors.},
  author={Claire McWhirter and Michael Tonge and Helen Plant and Ian M. Hardern and Willem Nissink and Stephen T Durant},
  journal={Journal of biomolecular screening},
  year={2013},
  volume={18 5},
  pages={
          567-75
        }
}
Flap endonuclease-1 (FEN1) is a highly conserved metallonuclease and is the main human flap endonuclease involved in the recognition and cleavage of single-stranded 5' overhangs from DNA flap structures. The involvement of FEN1 in multiple DNA metabolism pathways and the identification of FEN1 overexpression in a variety of cancers has led to interest in FEN1 as an oncology target. In this article, we describe the development of a 1536-well high-throughput screening assay based on the change in… CONTINUE READING

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