Development of a high performance liquid chromatography method for the quantitative determination of bioactive triterpenoids in the extracts of Antrodia camphorata

  title={Development of a high performance liquid chromatography method for the quantitative determination of bioactive triterpenoids in the extracts of Antrodia camphorata},
  author={Yerra Koteswara Rao and Madamanchi Geethangili and Yew-Min Tzeng},
  journal={Analytical Methods},
A simple gradient high-performance liquid chromatography with diode array detection (HPLC-DAD) method was developed for the simultaneous determination of total ten triterpenoids in Antrodia camphorata (AC). Optimum separation was obtained with a J'sphere C18 column using gradient elution with 0.2% aqueous acetic acid and acetonitrile as the mobile phase, a flow rate of 0.8 mL min−1, detection wavelength at 248 nm and sample volume at 5 μL. This method produced linear responses for ten… 

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