Development of a high-dynamic range, GFP-based FRET probe sensitive to oxidative microenvironments.

@article{Kolossov2011DevelopmentOA,
  title={Development of a high-dynamic range, GFP-based FRET probe sensitive to oxidative microenvironments.},
  author={Vladimir L Kolossov and Bryan Q Spring and Robert M. Clegg and Jennifer Jo Henry and Anna Sokolowski and Paul J. A. Kenis and H. Rex Gaskins},
  journal={Experimental biology and medicine},
  year={2011},
  volume={236 6},
  pages={681-91}
}
We report the optimization of a novel redox-sensitive probe with enhanced dynamic range and an exceptionally well-positioned oxidative midpoint redox potential. The present work characterizes factors that contribute to the improved Förster resonance energy transfer (FRET) performance of this green fluorescent protein (GFP)-based redox sensor. The α-helical linker, which separates the FRET donor and acceptor, has been extended in the new probe and leads to a decreased FRET efficiency in the… CONTINUE READING

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