Development of a high-content screening assay to identify compounds interfering with the formation of the hepatitis C virus replication complex.

@article{Berke2010DevelopmentOA,
  title={Development of a high-content screening assay to identify compounds interfering with the formation of the hepatitis C virus replication complex.},
  author={Jan Martin Berke and Denis Fenistein and Frederik Pauwels and Rob Bobbaers and Oliver Lenz and Tse-I Lin and Eberhard Krausz and Gregory Fanning},
  journal={Journal of virological methods},
  year={2010},
  volume={165 2},
  pages={
          268-76
        }
}
The hepatitis C virus (HCV) replicates its genome on a membrane-associated replication complex. These complexes are represented by "dot-like" structures on the endoplasmic reticulum when standard fluorescence microscopy techniques are applied. To screen compound libraries for inhibitors interfering with the formation of the HCV replication complex independent of RNA replication, an image-based high-content screening assay was developed utilizing inducible expression of the HCV non-structural… CONTINUE READING
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