Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter.

@article{Wu2008DevelopmentOA,
  title={Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter.},
  author={Yi Lian Wu and Xiufang Pan and Sudha Puttur Mudumana and Hai Wang and Peck Wai Kee and Zhiyuan Gong},
  journal={Gene},
  year={2008},
  volume={408 1-2},
  pages={85-94}
}
In the present study, a zebrafish hsp27 promoter was isolated and used to develop heat shock inducible gfp transgenic zebrafish. The endogenous hsp27 mRNAs were constitutively expressed from 4 hpf and increased in several regions of brain, heart and somites in early embryogenesis until 24 hpf. Subsequently, the expression was reduced significantly but maintained in the heart and ears. Heat shock induced hsp27 mRNAs in the blastoderm from 6 hpf and later in somites, branchial arches and several… CONTINUE READING
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