Development of a haemolysin gene-based multiplex PCR for simultaneous detection of Vibrio campbellii, Vibrio harveyi and Vibrio parahaemolyticus.

@article{Haldar2010DevelopmentOA,
  title={Development of a haemolysin gene-based multiplex PCR for simultaneous detection of Vibrio campbellii, Vibrio harveyi and Vibrio parahaemolyticus.},
  author={Soumya Haldar and Sucharit Basu Neogi and Kazuhiro Kogure and Shruti Chatterjee and Nityananda Chowdhury and Atsushi Hinenoya and Masahiro Asakura and Shinji Yamasaki},
  journal={Letters in applied microbiology},
  year={2010},
  volume={50 2},
  pages={146-52}
}
AIM To develop a haemolysin (hly) gene-based species-specific multiplex PCR for simple and rapid detection of Vibrio campbellii, V. harveyi and V. parahaemolyticus. METHODS AND RESULTS The complete hly genes of three V. campbellii strains isolated from diseased shrimps were sequenced and species-specific PCR primers were designed based on these sequences and the registered hly gene sequences of Vibrio harveyi and Vibrio parahaemolyticus. Specificity and sensitivity of the multiplex PCR was… CONTINUE READING

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