Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype-specific 3' noncoding sequences.

@article{Houng2001DevelopmentOA,
  title={Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype-specific 3' noncoding sequences.},
  author={H S Houng and R Chung-Ming Chen and David W. Vaughn and Niranjan Kanesa-thasan},
  journal={Journal of virological methods},
  year={2001},
  volume={95 1-2},
  pages={19-32}
}
A fluorogenic reverse transcriptase-polymerase chain reaction (RT-PCR) system was developed for use as a rapid diagnostic test for determining dengue viremia. The dengue virus 3'-noncoding sequence was utilized to formulate serotype-specific RT-PCR assays for quantitative identification of the four different dengue virus serotypes. A generic RT primer set… CONTINUE READING