Development of a fluorescence polarization immunoassay for the routine detection of N-desmethylzopiclone in urine samples.


A polarization fluoroimmunoassay was developed for the detected of N-desmethylzopiclone in urine and the reagents were adapted for use on the Vitalab Eclair analyser. Therefore, N-fluoresceinthiocarbamyldesmethylzopiclone was synthesized as a fluorescent tracer and used in combination with an existing pool of antibodies, raised against the hemisuccinyl… (More)