Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.

@article{Kozich2013DevelopmentOA,
  title={Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.},
  author={James J Kozich and Sarah L. Westcott and Nielson T. Baxter and Sarah K. Highlander and Patrick D. Schloss},
  journal={Applied and environmental microbiology},
  year={2013},
  volume={79 17},
  pages={5112-20}
}
Rapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds of 16S rRNA gene fragments per study using clone libraries to the sequencing of millions of fragments per study using next-generation sequencing technologies from 454 and Illumina. As these technologies advance, it is critical to assess the strengths, weaknesses, and overall suitability of these platforms for the interrogation… CONTINUE READING
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