Development of a dot blot neutralizing assay for HHV-6 and HHV-7 using specific monoclonal antibodies.

@article{Tsukazaki1998DevelopmentOA,
  title={Development of a dot blot neutralizing assay for HHV-6 and HHV-7 using specific monoclonal antibodies.},
  author={Takashi Tsukazaki and Masaru Yoshida and Hikaru Namba and Manabu Yamada and Noriaki Shimizu and Shiro Nii},
  journal={Journal of virological methods},
  year={1998},
  volume={73 2},
  pages={141-9}
}
To elucidate further immune responses to human herpesviruses 6 and 7 (HHV-6 and -7), a neutralizing antibody assay was established for these viruses using a dot blot method. Three monoclonal antibodies against HHV-6 and 12 monoclonal antibodies against HHV-7 were developed and characterized by radio-immunoprecipitation. One monoclonal antibody which recognizes the 135 kDa late polypeptide of HHV-6 and several which recognize the 125 kDa late polypeptide of HHV-7 were selected to monitor virus… CONTINUE READING