Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein

@inproceedings{Fu2014DevelopmentOA,
  title={Development of a direct competitive ELISA for the detection of Mycoplasma bovis infection based on a monoclonal antibody of P48 protein},
  author={P. L. Fu and Zhenhong Sun and Yuewei Zhang and Ziqiang Yu and H Zhang and Dan Su and Fei Jiang and Wenxue Wu},
  booktitle={BMC veterinary research},
  year={2014}
}
BACKGROUND Mycoplasma bovis (M. bovis) is a major, but often overlooked, pathogen documented to cause respiratory disease, mastitis, and arthritis in cattle throughout China since 2008. Here, we report the development of a direct competitive enzyme-linked immunosorbent assay (Dc-ELISA) to detect M. bovis antibody. RESULTS We used a recombinant P48 protein and monoclonal antibody (mAb) 10E. MAb 10E, prepared against the recombinant P48 protein of M. bovis, identified all M. bovis strains with… CONTINUE READING
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