Development of a direct assay for alpha-amylase.


We describe a direct colorimetric assay for alpha-amylase, with 2-chloro-4-nitrophenyl-alpha-maltotrioside as substrate. Both human pancreatic and salivary amylase split this substrate without the use of helper enzymes, yielding free 2-chloro-4-nitrophenol, which is monitored at 405 nm. The performance of this reagent compares well with that of Pantrak… (More)


Cite this paper

@article{WinnDeen1988DevelopmentOA, title={Development of a direct assay for alpha-amylase.}, author={E. Winn-Deen and H. D{\'a}vid and Gary R. Sigler and R. I. Mata Chavez}, journal={Clinical chemistry}, year={1988}, volume={34 10}, pages={2005-8} }