Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test.

@article{Jeune1995DevelopmentOA,
  title={Development of a detection system for histidine decarboxylating lactic acid bacteria based on DNA probes, PCR and activity test.},
  author={Christine Le Jeune and Aline Lonvaud-Funel and B J E ten Brink and Harmen Hofstra and Jos van der Vossen},
  journal={The Journal of applied bacteriology},
  year={1995},
  volume={78 3},
  pages={
          316-26
        }
}
On the basis of the comparison of the nucleotide sequences of the histidine decarboxylase genes (hdcA) of Lactobacillus 30A and Clostridium perfringens and the amino acid sequences of these histidine decarboxylases and those of Lactobacillus buchneri and Micrococcus, oligonucleotides unique to the hdcA genes were synthesized and used in PCR. All histidine-decarboxylating lactic acid bacteria gave a signal with primer set JV16HC/JV17HC in PCR. In addition to this primer set, CL1/CL2 and CL1… CONTINUE READING

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